Part:BBa_K3794000

PVFP-5

Perna viridis foot protein-5 (PVFP-5), is one of the first adhesive proteins secreted by the mussel foot from the Perna viridis mussel species.

PVFP-5 is an adhesive protein that can be utilised in biomedical and therapeutic applications due to its low immunogenicity and non-cytotoxicity in the body. It can be used as a bioadhesive coating for medical implants, such as a 3D printed scaffold / nerve channel for axonal regeneration.

There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.

This part has been synthesised by IDT as a composite, which can be found at BBa_K3794001 (with an upstream LacI Regulator (BBa_R0010)).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]